Control:Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PML antibody Bethyl Laboratories Cat# A301-167A; RRID:AB_873108 PML antibody Santa Cruz Biotechnology Cat# sc-966; RRID:AB_628162 SP100 antibody Abcam Cat# ab167605; RRID: AB_3716604 ATRX antibody Cell Signaling Technology Cat# 10321; RRID: AB_2940903 SETDB1 antibody Abcam Cat# ab107225; RRID: AB_10861045 DAXX antibody Cell Signaling Technology Cat# 4533; RRID: AB_2088778 HP1α antibody Abcam Cat# ab77256; RRID: AB_1523784 Myc-tag antibody Cell Signaling Technology Cat# 2276; RRID: AB_331783 β-Actin antibody Cell Signaling Technology Cat# 8457; RRID: AB_10950489 H3K9me3 antibody Abcam Cat# ab8898; RRID: AB_306848 Histone H3 antibody Abcam Cat# ab1791; RRID: AB_302613 IgG control antibody Abcam Cat# ab37415 TRF2 antibody Novus Biologicals Cat# NB110-57130; RRID: AB_844199 BLM antibody Bethyl Laboratories, Thermo Fisher Cat# A300-110A; RRID: AB_2779016 Anti-Digoxigenin-AP, Fab fragments Roche Cat# 11093274910; RRID: AB_514497 Anti-mouse HRP Cell Signaling Technology Cat# 7076; RRID: AB_330924 Anti-rabbit HRP Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Alexa Fluor 568 goat anti-rabbit IgG Invitrogen Cat# A11036; RRID: AB_10563566 Alexa Fluor 568 goat anti-mouse IgG Invitrogen Cat# A11031; RRID: AB_144696 Alexa Fluor 568 goat anti-rabbit IgG Invitrogen Cat# A11008; RRID: AB_143165 Alexa Fluor 488 goat anti-mouse IgG Invitrogen Cat# A11001; RRID: AB_2534069 Alexa Fluor 647 goat anti-mouse IgG Invitrogen Cat# A21236; RRID: AB_2535805 Bacterial and virus strains NEB Stable New England Biolabs C3040 Chemicals, peptides, and recombinant proteins Blasticidin RPI B12200–0.05 Polybrene Santa Cruz Biotechnology Cat# sc-134220 DMEM Corning Cat# 10-013CV Carbenecillin IBI Scientific 3P IB02025 Colcemid Gibco KaryoMAX 15212–012 Doxycycline TCI D4116 RO3306 CDK1 inhibitor Millipore Sigma Cat# SML0569 Fluoroshield with DAPI Sigma-Aldrich F6057 HEPES GIBCO 15630080 MEM Nonessential Amino Acids Corning 25-025CI Penicilin/Streptomycin Lonza 09-757F Puromycin Alfa Aesar 61278-MC NEBNext Ultra II Q5 Master Mix NEB M0544X Triton X- MP Biomedicals 9002-93-1 PEI Max Polysciences Inc. 24765–1 HyClone Cosmic Calf Serum Cytiva SH30087.04 Thymidine Millipore Sigma Cat# T9250 SUMO inhibitor (SUMOi) Selleck Chemical Cat# S8697 Opti-MEM I Gibco Cat# 31985-070 (Continued on next page) 20 Cell Reports 45, 117004, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778030 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000015 HMW Poly(I:C) InvivoGen Cat# tlrl-pic Protease QIAGEN Cat# 19157 Benzonase Nuclease EMD Millipore Cat# 70746-3 Formamide Millipore Sigma Cat# S4117 Blocking reagent Roche Cat# 11096176001 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Acros Organics Cat# 32711500 Chloroform Fisher Chemical Cat# C574-1 100% Ethanol Decon Laboratories, Inc. Cat# 2701 Sodium Acetate VWR Cat# 0602-500G HinfI restriction enzyme New England Biolabs Cat# R0155L RsaI restriction enzyme New England Biolabs Cat# R0167 Φ29 Reaction Buffer New England Biolabs Cat# B0269S dNTP mix Qiagen Cat# 201913 Φ29 DNA Polymerase New England Biolabs Cat# M0629L CDP-Star Roche Cat# 11759051001 Pepsin Fisher Scientific Cat# A104-500 Paraformaldehyde, 16% w/v aq soln., methanol free Thermo Scientific Cat. No: 043368.9 RNase A Thermo Scientific Ref# EN0531 Protein A/G Magnetic Beads Thermo Scientific Cat# 88802 Protease inhibitor cocktail Roche Cat# 05892791001 ChIP protease inhibitor mix Diagenode Cat# C12010011 Nucleofection buffer MaxCyte Cat# EPB-1 Cas9 protein IDT Cat# 1081060 Neomycin Thermo Scientific J62671.03 Critical commercial assays BCA Protein Assay ThermoFischer 23227 DIG Oligonucleotide 3 ′ End Labeling Kit, 2ND generation Roche Cat# 03353575910 Qubit High Sensitivity dsDNA Kit Invitrogen Q32851 Click-iT EdU Cell Proliferation Kit for Imaging Thermo Fisher Scientific Cat# C10340 Qiagen DNA Purification Kit Qiagen Cat# 28106 DNA Clean and Concentrator 5-G Zymo Research D4013 Infusion Takara 638948 Zymoclean Gel DNA Recovery Kit Zymo Research D4001 Super Signal West Dura Genesee Scientific 20–301 Super Signal West Femto Genesee Scientific 20–302 Amersham Hybond P 0.2μm PVDF Cytiva 10600021 Bolt 4%–12% Bis-Tris Plus gels Invitrogen NW04120 Nucleofection Buffer MaxCyte EPB-1 Electroporation cuvette MaxCyte SOC-25x3 Experimental models: Cell lines
Virus:Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PML antibody Bethyl Laboratories Cat# A301-167A; RRID:AB_873108 PML antibody Santa Cruz Biotechnology Cat# sc-966; RRID:AB_628162 SP100 antibody Abcam Cat# ab167605; RRID: AB_3716604 ATRX antibody Cell Signaling Technology Cat# 10321; RRID: AB_2940903 SETDB1 antibody Abcam Cat# ab107225; RRID: AB_10861045 DAXX antibody Cell Signaling Technology Cat# 4533; RRID: AB_2088778 HP1α antibody Abcam Cat# ab77256; RRID: AB_1523784 Myc-tag antibody Cell Signaling Technology Cat# 2276; RRID: AB_331783 β-Actin antibody Cell Signaling Technology Cat# 8457; RRID: AB_10950489 H3K9me3 antibody Abcam Cat# ab8898; RRID: AB_306848 Histone H3 antibody Abcam Cat# ab1791; RRID: AB_302613 IgG control antibody Abcam Cat# ab37415 TRF2 antibody Novus Biologicals Cat# NB110-57130; RRID: AB_844199 BLM antibody Bethyl Laboratories, Thermo Fisher Cat# A300-110A; RRID: AB_2779016 Anti-Digoxigenin-AP, Fab fragments Roche Cat# 11093274910; RRID: AB_514497 Anti-mouse HRP Cell Signaling Technology Cat# 7076; RRID: AB_330924 Anti-rabbit HRP Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Alexa Fluor 568 goat anti-rabbit IgG Invitrogen Cat# A11036; RRID: AB_10563566 Alexa Fluor 568 goat anti-mouse IgG Invitrogen Cat# A11031; RRID: AB_144696 Alexa Fluor 568 goat anti-rabbit IgG Invitrogen Cat# A11008; RRID: AB_143165 Alexa Fluor 488 goat anti-mouse IgG Invitrogen Cat# A11001; RRID: AB_2534069 Alexa Fluor 647 goat anti-mouse IgG Invitrogen Cat# A21236; RRID: AB_2535805 Bacterial and virus strains NEB Stable New England Biolabs C3040 Chemicals, peptides, and recombinant proteins Blasticidin RPI B12200–0.05 Polybrene Santa Cruz Biotechnology Cat# sc-134220 DMEM Corning Cat# 10-013CV Carbenecillin IBI Scientific 3P IB02025 Colcemid Gibco KaryoMAX 15212–012 Doxycycline TCI D4116 RO3306 CDK1 inhibitor Millipore Sigma Cat# SML0569 Fluoroshield with DAPI Sigma-Aldrich F6057 HEPES GIBCO 15630080 MEM Nonessential Amino Acids Corning 25-025CI Penicilin/Streptomycin Lonza 09-757F Puromycin Alfa Aesar 61278-MC NEBNext Ultra II Q5 Master Mix NEB M0544X Triton X- MP Biomedicals 9002-93-1 PEI Max Polysciences Inc. 24765–1 HyClone Cosmic Calf Serum Cytiva SH30087.04 Thymidine Millipore Sigma Cat# T9250 SUMO inhibitor (SUMOi) Selleck Chemical Cat# S8697 Opti-MEM I Gibco Cat# 31985-070 (Continued on next page) 20 Cell Reports 45, 117004, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778030 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000015 HMW Poly(I:C) InvivoGen Cat# tlrl-pic Protease QIAGEN Cat# 19157 Benzonase Nuclease EMD Millipore Cat# 70746-3 Formamide Millipore Sigma Cat# S4117 Blocking reagent Roche Cat# 11096176001 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Acros Organics Cat# 32711500 Chloroform Fisher Chemical Cat# C574-1 100% Ethanol Decon Laboratories, Inc. Cat# 2701 Sodium Acetate VWR Cat# 0602-500G HinfI restriction enzyme New England Biolabs Cat# R0155L RsaI restriction enzyme New England Biolabs Cat# R0167 Φ29 Reaction Buffer New England Biolabs Cat# B0269S dNTP mix Qiagen Cat# 201913 Φ29 DNA Polymerase New England Biolabs Cat# M0629L CDP-Star Roche Cat# 11759051001 Pepsin Fisher Scientific Cat# A104-500 Paraformaldehyde, 16% w/v aq soln., methanol free Thermo Scientific Cat. No: 043368.9 RNase A Thermo Scientific Ref# EN0531 Protein A/G Magnetic Beads Thermo Scientific Cat# 88802 Protease inhibitor cocktail Roche Cat# 05892791001 ChIP protease inhibitor mix Diagenode Cat# C12010011 Nucleofection buffer MaxCyte Cat# EPB-1 Cas9 protein IDT Cat# 1081060 Neomycin Thermo Scientific J62671.03 Critical commercial assays BCA Protein Assay ThermoFischer 23227 DIG Oligonucleotide 3 ′ End Labeling Kit, 2ND generation Roche Cat# 03353575910 Qubit High Sensitivity dsDNA Kit Invitrogen Q32851 Click-iT EdU Cell Proliferation Kit for Imaging Thermo Fisher Scientific Cat# C10340 Qiagen DNA Purification Kit Qiagen Cat# 28106 DNA Clean and Concentrator 5-G Zymo Research D4013 Infusion Takara 638948 Zymoclean Gel DNA Recovery Kit Zymo Research D4001 Super Signal West Dura Genesee Scientific 20–301 Super Signal West Femto Genesee Scientific 20–302 Amersham Hybond P 0.2μm PVDF Cytiva 10600021 Bolt 4%–12% Bis-Tris Plus gels Invitrogen NW04120 Nucleofection Buffer MaxCyte EPB-1 Electroporation cuvette MaxCyte SOC-25x3 Experimental models: Cell lines
Recombinant:Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PML antibody Bethyl Laboratories Cat# A301-167A; RRID:AB_873108 PML antibody Santa Cruz Biotechnology Cat# sc-966; RRID:AB_628162 SP100 antibody Abcam Cat# ab167605; RRID: AB_3716604 ATRX antibody Cell Signaling Technology Cat# 10321; RRID: AB_2940903 SETDB1 antibody Abcam Cat# ab107225; RRID: AB_10861045 DAXX antibody Cell Signaling Technology Cat# 4533; RRID: AB_2088778 HP1α antibody Abcam Cat# ab77256; RRID: AB_1523784 Myc-tag antibody Cell Signaling Technology Cat# 2276; RRID: AB_331783 β-Actin antibody Cell Signaling Technology Cat# 8457; RRID: AB_10950489 H3K9me3 antibody Abcam Cat# ab8898; RRID: AB_306848 Histone H3 antibody Abcam Cat# ab1791; RRID: AB_302613 IgG control antibody Abcam Cat# ab37415 TRF2 antibody Novus Biologicals Cat# NB110-57130; RRID: AB_844199 BLM antibody Bethyl Laboratories, Thermo Fisher Cat# A300-110A; RRID: AB_2779016 Anti-Digoxigenin-AP, Fab fragments Roche Cat# 11093274910; RRID: AB_514497 Anti-mouse HRP Cell Signaling Technology Cat# 7076; RRID: AB_330924 Anti-rabbit HRP Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Alexa Fluor 568 goat anti-rabbit IgG Invitrogen Cat# A11036; RRID: AB_10563566 Alexa Fluor 568 goat anti-mouse IgG Invitrogen Cat# A11031; RRID: AB_144696 Alexa Fluor 568 goat anti-rabbit IgG Invitrogen Cat# A11008; RRID: AB_143165 Alexa Fluor 488 goat anti-mouse IgG Invitrogen Cat# A11001; RRID: AB_2534069 Alexa Fluor 647 goat anti-mouse IgG Invitrogen Cat# A21236; RRID: AB_2535805 Bacterial and virus strains NEB Stable New England Biolabs C3040 Chemicals, peptides, and recombinant proteins Blasticidin RPI B12200–0.05 Polybrene Santa Cruz Biotechnology Cat# sc-134220 DMEM Corning Cat# 10-013CV Carbenecillin IBI Scientific 3P IB02025 Colcemid Gibco KaryoMAX 15212–012 Doxycycline TCI D4116 RO3306 CDK1 inhibitor Millipore Sigma Cat# SML0569 Fluoroshield with DAPI Sigma-Aldrich F6057 HEPES GIBCO 15630080 MEM Nonessential Amino Acids Corning 25-025CI Penicilin/Streptomycin Lonza 09-757F Puromycin Alfa Aesar 61278-MC NEBNext Ultra II Q5 Master Mix NEB M0544X Triton X- MP Biomedicals 9002-93-1 PEI Max Polysciences Inc. 24765–1 HyClone Cosmic Calf Serum Cytiva SH30087.04 Thymidine Millipore Sigma Cat# T9250 SUMO inhibitor (SUMOi) Selleck Chemical Cat# S8697 Opti-MEM I Gibco Cat# 31985-070 (Continued on next page) 20 Cell Reports 45, 117004, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778030 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000015 HMW Poly(I:C) InvivoGen Cat# tlrl-pic Protease QIAGEN Cat# 19157 Benzonase Nuclease EMD Millipore Cat# 70746-3 Formamide Millipore Sigma Cat# S4117 Blocking reagent Roche Cat# 11096176001 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Acros Organics Cat# 32711500 Chloroform Fisher Chemical Cat# C574-1 100% Ethanol Decon Laboratories, Inc. Cat# 2701 Sodium Acetate VWR Cat# 0602-500G HinfI restriction enzyme New England Biolabs Cat# R0155L RsaI restriction enzyme New England Biolabs Cat# R0167 Φ29 Reaction Buffer New England Biolabs Cat# B0269S dNTP mix Qiagen Cat# 201913 Φ29 DNA Polymerase New England Biolabs Cat# M0629L CDP-Star Roche Cat# 11759051001 Pepsin Fisher Scientific Cat# A104-500 Paraformaldehyde, 16% w/v aq soln., methanol free Thermo Scientific Cat. No: 043368.9 RNase A Thermo Scientific Ref# EN0531 Protein A/G Magnetic Beads Thermo Scientific Cat# 88802 Protease inhibitor cocktail Roche Cat# 05892791001 ChIP protease inhibitor mix Diagenode Cat# C12010011 Nucleofection buffer MaxCyte Cat# EPB-1 Cas9 protein IDT Cat# 1081060 Neomycin Thermo Scientific J62671.03 Critical commercial assays BCA Protein Assay ThermoFischer 23227 DIG Oligonucleotide 3 ′ End Labeling Kit, 2ND generation Roche Cat# 03353575910 Qubit High Sensitivity dsDNA Kit Invitrogen Q32851 Click-iT EdU Cell Proliferation Kit for Imaging Thermo Fisher Scientific Cat# C10340 Qiagen DNA Purification Kit Qiagen Cat# 28106 DNA Clean and Concentrator 5-G Zymo Research D4013 Infusion Takara 638948 Zymoclean Gel DNA Recovery Kit Zymo Research D4001 Super Signal West Dura Genesee Scientific 20–301 Super Signal West Femto Genesee Scientific 20–302 Amersham Hybond P 0.2μm PVDF Cytiva 10600021 Bolt 4%–12% Bis-Tris Plus gels Invitrogen NW04120 Nucleofection Buffer MaxCyte EPB-1 Electroporation cuvette MaxCyte SOC-25x3 Experimental models: Cell lines
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